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KMID : 0545120080180061141
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 6 p.1141 ~ p.1145
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Optimized Substrate Concentrations for Production of Long-Chain Isomaltooligosaccharides Using Dextransucrase of Leuconostoc mesenteroides B-512F
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Lee Min-Sung
Cho Seung-Kee
Eom Hyun-Ju
Kim So-Young
Kim Tae-Jip
Han Nam-Soo
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Abstract
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Isomaltooligosaccharide (IMO) is a promising dietary component with prebiotic effect, and the long-chain IMOs are preferred to short chain ones owing to the longer persistence in the colon. To establish the optimal process for synthesis of long-chain IMOs, we systematically examined the reaction condition of dextransucrase of Leuconostoc mesenteroides B-512F by changing the ratio of sucrose to maltose (varying as 1:4, 1:2, 1:1, and 2:1) and amount of each sugar (from 2% to 20%). As a result, a ratio of 2:1 (sucrose to maltose, 10:5% or 20:10%, w/v) was determined as an optimal condition for long-chain IMO synthesis (DP3-DP9) with relatively higher yields (70-90%, respectively).
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KEYWORD
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Leuconostoc mesenteroides, dextransucrase, isomaltooligosaccharide, prebiotic, acceptor reaction
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